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A validated high-performance liquid chromatography method for the determination of rifampicin and desacetyl rifampicin in plasma and urine

Hemanth Kumar, A K and Chandra, I and Geetha, R and Silambu Chelvi, K and Lalitha, V and Prema, G (2004) A validated high-performance liquid chromatography method for the determination of rifampicin and desacetyl rifampicin in plasma and urine. Indian Journal of Pharmacology, 36 (4). pp. 231-233. ISSN 0253-7613

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Abstract

Objective: To standardize a high-performance liquid chromatography (HPLC) method for the determination of rifampicin (RMP) and its major metabolite desacetyl rifampicin (DRMP) in plasma and urine. Material and Methods: A simple, specific and sensitive HPLC method was developed for the determination of RMP and DRMP in plasma and urine. Separation in both was achieved by reversephase chromatography on a C18 column with a mobile phase composition of 0.05 M phosphate buffer: acetonitrile (55:45 v/v) at 254 nm. Results: The retention times of DRMP, RMP and Rifapentine (RPN), the internal standard were 2.9, 4.8 and 10.5 min respectively. The assay was linear from 0.25 to 15.0 μg ml-1 for plasma and 2.5 to 80.0 μg ml-1 for urine. Both intra-day and inter-day accuracy and precision data showed good reproducibility. Conclusion: The HPLC method described is sensitive, selective and linear for the wide range of concentrations for RMP and DRMP in plasma and urine. Thus, the method developed is well suited for the pharmacokinetic studies.

NIRT Creators:
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Item Type: Article
Uncontrolled Keywords: Rifampin, rifamycin, HPLC
Subjects: Tuberculosis > Laboratory Research > Biochemical,Clinical and Pharmacological
Divisions: Basic Science Research > Biochemistry and Pharmacology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 20 Nov 2013 05:19
Last Modified: 09 Mar 2016 10:18
URI: http://eprints.nirt.res.in/id/eprint/643

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