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Elicitation of efficient, protective immune responses by using DNA vaccines against tuberculosis

Khera, A and Singh, R and Shakila, H and Rao, V and Dhar, N and Narayanan, P R and Paramasivan, C N and Ramanathan, V D and Tyagi, A K (2005) Elicitation of efficient, protective immune responses by using DNA vaccines against tuberculosis. Vaccine, 23 (48-49). pp. 5655-5665. ISSN 0264-410X

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DNA vaccination is an effective method for elicitation of strong humoral as well as cellular immune responses. DNA vaccines expressing mycobacterial antigens ESAT-6 (Rv3875), �-crystallin (Rv2031c) and superoxide dismutase A (Rv3846) were evaluated for their immune responses in Balb/c mice and protective efficacy in guinea pigs. Immunization of mice with theDNAvaccines expressing superoxide dismutase Aand �-crystallin resulted in markedly higher levels of IFN-� as compared to the levels of IL-10. TheDNAvaccine expressing ESAT-6 elicited a mixed Th1/Th2 response. Immunization of guinea pigs with these DNA vaccines and subsequent challenge of animals with Mycobacterium tuberculosis H37Rv, showed that DNA vaccine expressing superoxide dismutase imparted the maximum protection as observed by a 50 and 10 folds reduction in bacillary load in spleens and lungs, respectively, in comparison to immunization with vector control.

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Item Type: Article
Uncontrolled Keywords: DNA vaccines; Tuberculosis; Immune response
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 27 Nov 2013 09:47
Last Modified: 09 Mar 2016 07:07

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