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Simple and rapid liquid chromatography method for determination of efavirenz in plasma

Ramachandran, Geetha and Hemanth Kumar, A K and Swaminathan, Soumya and Venkatesan, P and Kumaraswami, V and Greenblatt, D J (2006) Simple and rapid liquid chromatography method for determination of efavirenz in plasma. Journal of Chromatography B, 835 (1-2). pp. 131-135. ISSN 1570-0232

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Abstract

A simple and rapid high performance liquid chromatographic method for determination of efavirenz in human plasma was developed. The method involved extraction of sample with ethyl acetate and analysis using a reversed-phase C18 column (150 mm) with UV detection. The assay was linear from 0.0625 to 10.0 �g/ml. The method was specific for efavirenz estimation and the drug was stable in plasma up to one month at −20 ◦C. The average recovery of efavirenz from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring of efavirenz.

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Item Type: Article
Uncontrolled Keywords: Efavirenz; Plasma; HPLC
Subjects: Tuberculosis > Laboratory Research > Biochemical,Clinical and Pharmacological
Divisions: Basic Science Research > Biochemistry and Pharmacology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 03 Dec 2013 11:17
Last Modified: 09 Mar 2016 06:20
URI: http://eprints.nirt.res.in/id/eprint/744

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