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Elevated percentage of perforin positive cells in active pulmonary tuberculosis

Nisha Rajeswari, D and Selvaraj, P and Jawahar, M S and Ashilakshmi, A R and Vidyarani, M and Narayanan, P R (2006) Elevated percentage of perforin positive cells in active pulmonary tuberculosis. Indian Journal of Medical Research, 123 (May). pp. 687-690. ISSN 0971-5916

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Background & objectives: Perforin is one of the major effector molecules of cytotoxic cells associated with killing of cells harbouring intracellular bacterial infection. The precise role of perforin positive cells in tuberculosis still remains controversial. The present study was done to determine the number of circulating CD4+ and CD8+ perforin positive cells to assess the level of cytotoxic response against Mycobacterium tuberculosis in patients with pulmonary tuberculosis. Methods: Intracellular perforin and surface CD4 and CD8 staining of peripheral blood lymphocytes was done using specific monoclonal antibodies and enumerated using flowcytometry. Results: A significantly decreased total lymphocytes (P<0.01), CD4 (P<0.001) and CD8 (P<0.01) lymphocyte counts in PTB patients was observed compared to normal healthy individuals (NHS). Intracellular perforin staining showed significantly elevated percentages of total (P<0.05) and CD8 (P<0.01) perforin positive cells in PTB patients compared to NHS. However, the absolute counts of total, CD4 and CD8 cells positive for perforin were similar in patients and NHS. Interpretation & conclusion: Our results suggest that during active stage of pulmonary tuberculosis there was an increased percentage of CD8 cells positive for perforin, irrespective of their absolute counts. Further, CD8+ perforin positive cells may

Item Type: Article
Uncontrolled Keywords: CD4, CD8, perforin positive cells, pulmonary tuberculosis
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 12 Dec 2013 06:32
Last Modified: 09 Mar 2016 06:14

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