Rajavelu, Priya and Madhumathi, J and Das, Sulochana D
(2006)
Humoral immune responses of normals
and tuberculosis patients to multiple
sonicate antigens prepared from
the most prevalent strains of
Mycobacterium tuberculosis
harbouring single copy of IS6110
from South India.
Current Science, 91 (7).
pp. 918-922.
ISSN 0011-3891
Abstract
The search for newer species-specific antigens from
prevalent strains of Mycobacterium tuberculosis (Mtb)
associated with active disease will add substantially to
improve the presently available diagnostic tools. We
studied the protein profiles of 13 sonicate antigens (S1–
S13) prepared from the prevalent strains. The humoral
immune response was also studied in 30 normal
and 30 tuberculosis (TB) patients. The sonicate antigens
S10 and S12 showed maximum differential protein
bands in low molecular mass region of 10–30 kDa on
SDS–PAGE. Our ELISA results showed significant
increase in Mtb-specific IgG antibodies in TB plasma
for H37Rv, followed by PPD, S1 and S10 antigens.
Immunoblot analysis of S10 antigen showed specific
recognition pattern at low molecular mass region by TB
plasma alone with 12–77% positivity of protein bands.
Thus the sonicate antigen S10 was found to be discriminatory
by ELISA and Western blot and hence a
good candidate for further purification of its individual
proteins to be evaluated for diagnosis.
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