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Identification & differentiation of Mycobacterium avium & M. intracellulare by PCR- RFLP assay using the groES gene

Aravindhan, V and Sulochana, S and Narayanan, Sujatha and Paramasivan, C N and Narayanan, P R (2007) Identification & differentiation of Mycobacterium avium & M. intracellulare by PCR- RFLP assay using the groES gene. Indian Journal of Medical Research, 126 (Dec). pp. 575-579. ISSN 0971-5916

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Abstract

Background & objectives: We report a new polymerase chain reaction (PCR) – restriction fragment length polymorphism (RFLP) assay using mycobacterial groES as a target to identify Mycobacterium avium and M. intracellulare in clinical samples. Methods: The assay was standardized using M. avium and M. intracellulare standard strains obtained from ATCC and was tested with 45 M. avium-M. intracellulare complex (MAC) clinical isolates (Of which 31 were from HIV+ individuals). The standard and clinical strains were typed with HPLC based mycolic acid fingerprinting. Results: Three polymorphisms (BamHI, BstNI and HgaI) were identified for inter-species differentiation among standard strains; of which, only HgaI was found to be useful in clinical isolates. Of the 45 isolates, 25 were M. avium and 20 were M. intracelluare. MAC isolates, which could not be differentiated by HPLC analysis, were also typed by this method. Interpretation & conclusions: The use of mycobacterial groES as a PCR-RFLP target for M. avium and M. intracellulare is a simple and rapid method that can complement HPLC in their differentiation.

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Item Type: Article
Uncontrolled Keywords: groES, HPLC, MAC, Mycobacterium avium, M. intracellulare, PCR-RFLP
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 31 Dec 2013 10:26
Last Modified: 09 Mar 2016 04:32
URI: http://eprints.nirt.res.in/id/eprint/794

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