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Evaluation of the diagnostic potential of region of deletion-1–encoded antigen culture filtrate protein-10 in pulmonary tuberculosis

Murthy, Madhan Kumar and Parasa, Ramana Rao V and Deenadayalan , Anbarasu and Sharma, P and Raja, Alamelu (2007) Evaluation of the diagnostic potential of region of deletion-1–encoded antigen culture filtrate protein-10 in pulmonary tuberculosis. Diagnostic Microbiology and Infectious Disease, 59 (3). pp. 295-302. ISSN 0732-8893

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Abstract

The ability of region of deletion-1 (RD-1)–encoded culture filtrate protein-10 (CFP-10) to supplement the sensitivity of 38-kDa antigen was studied using enzyme-linked immunosorbent assay in pulmonary tuberculosis (TB) patients and controls. The sensitivities for individual antigens ranged from 50% to 60%, and the specificity was 100% for immunoglobulin (Ig) G alone. When IgA results were added to IgG, the sensitivity increased. On combination of the isotype results, sensitivity increased to 61.8% and 57.2% (for 38-kDa antigen and CFP-10), respectively, and specificity changed to 98.8% and 99.4% for 38-kDa and CFP-10, respectively. Combination of results of both the antigens gave 82.4% sensitivity in smear-positive and culture-positive group, and 98.1% specificity. In smear-negative and culture-positive group, the sensitivity was 66.7%. In smear-negative and culture-negative cases, a sensitivity of 65.6% was obtained. This study demonstrates that the use of RD-1–encoded CFP-10 enhances the sensitivity of 38-kDa antigen and can be a useful diagnostic marker in TB.

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Item Type: Article
Uncontrolled Keywords: RD-1; Serodiagnosis; CFP-10 antigen; 38-kDa antigen; Pulmonary tuberculosis; ELISA
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 08 Jan 2014 10:52
Last Modified: 09 Mar 2016 04:42
URI: http://eprints.nirt.res.in/id/eprint/810

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