Blackwell Publishing Ltd Influence of HLA-DR2 on perforin-positive cells in pulmonary tuberculosis

Rajeswari, D N and Selvaraj, P and Raghavan, S and Jawahar, M S and Narayanan, P R (2007) Blackwell Publishing Ltd Influence of HLA-DR2 on perforin-positive cells in pulmonary tuberculosis. International Journal of Immunogenetics, 34 (5). pp. 379-384. ISSN Print: 1744-3121; Online: 1744-313X

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Perforin is one of the key effector molecules of cytotoxic T cells and natural killer cells. The influence of HLADRB1 alleles on peripheral blood perforin-positive CD4, CD8, CD16 and CD56 cells was studied by flow cytometry. HLA-DRB1 typing was done in normal healthy subjects (NHS: n = 156) and patients with pulmonary tuberculosis (PTB: n = 102) by polymerase chain reaction-based sequence-specific oligonucleotide hybridization method. We observed a significantly decreased percentage of total perforin-positive cells (per+) (P = 0.0004); CD8+/Per+ (P = 0.0005); CD16+/Per+ (P = 0.05) and CD56+/Per+ cells (P = 0.001) in HLA-DR2-positive PTB patients compared to non-DR2 patients. Subtyping of HLA-DR2-positive subjects at the allelic level revealed that the percentage of CD8+/Per+ cells did not differ among DRB1*1501 and DRB1*1502 patients while a trend towards a decreased percentage of CD16+/Per+ and CD56+/Per+ cells was noticed in DRB1*1501 patients compared to DRB1*1502 patients. The present study suggests that HLA-DR2 may be associated with down-regulation of perforin-positive cytotoxic lymphocytes and natural killer cells in pulmonary tuberculosis.

Item Type: Article
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 09 Jan 2014 08:37
Last Modified: 30 Mar 2021 04:56

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