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Cytokine gene polymorphisms and cytokine levels in pulmonary tuberculosis

Selvaraj, P and Alagarasu, K and Harishankar, M and Vidyarani, M and Nisha Rajeswari, D and Narayanan, P R (2008) Cytokine gene polymorphisms and cytokine levels in pulmonary tuberculosis. Cytokine, 43 (1). pp. 26-33. ISSN 1043-4666; eISSN: 1096-0023

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Abstract

Polymorphisms in the cytokine genes are known to influence cytokine levels and may be associated with outcome of infections. We investigated the polymorphisms in the cytokine genes namely IFN-c (+874 and +5644), IL-2 (�330 and +160), IL-4 (VNTR), IL-6 (�174), IL-10 (�1082 and �819) and IL-12B (+1188) in 188 normal healthy subjects (NHS) and 166 pulmonary tuberculosis patients (PTB) using polymerase chain reaction-based methods. To study the influence of cytokine gene polymorphisms on cytokine levels, phytohaemagglutinin and culture filtrate antigen of Mycobacterium tuberculosis-induced cytokine levels were measured by ELISA from 72-h-old peripheral blood mononuclear cell culture supernatants. Significantly decreased frequency of TT genotype of IL-2 �330 polymorphism (p = 0.024, odds ratio (OR) 0.53, 95% CI 0.31–0.92) was observed in patients compared to NHS. The genotype frequencies of other polymorphisms were not different between patients and NHS. IL-12p40 levels were significantly decreased among NHS with AA genotype of IL-12B gene polymorphism compared to NHS with AC genotype (p < 0.05). Increased levels of IL-12p40 were observed among patients with CC genotype of IL-12B gene compared to patients with other genotypes (p < 0.01). The present study suggests that the TT genotype of IL-2 �330 polymorphism may be associated with the protection to PTB in south India. Further, +1188 polymorphism of IL-12B gene either alone or in combination with closely linked genes may regulate IL-12p40 production and may play a major role on acquired immunity to tuberculosis.

NIRT Creators:
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Item Type: Article
Uncontrolled Keywords: Tuberculosis Gene polymorphisms Cytokines
Subjects: Tuberculosis > Laboratory Research > Immunological
Tuberculosis
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 21 Jul 2017 09:28
Last Modified: 21 Jul 2017 11:32
URI: http://eprints.nirt.res.in/id/eprint/892

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