Madhavan, J and Mitra, M
(2009)
KIF14 and E2F3 mRNA expression in human retinoblastoma and
its phenotype association.
Moleclar Vision, 15.
pp. 235-240.
ISSN : 1090-0535, eISSN: 1090-0535
Abstract
Purpose: We quantified mRNA expression of candidate genes for proliferation (KIF14 and E2F3) in a large
retinoblastoma tumor cohort and associated with disease phenotype.
Methods: KIF14 and E2F3 mRNA expression was quantified by real time PCR in 57 retinoblastoma (RB) tumors, 3 RB
cell lines, and control samples that included 4 each fetal, age-matched, adult retinas. Immunohistochemistry was done to
confirm KIF14 and E2F3 protein expression in tumor cells. The mRNA expression levels were correlated with disease
phenotypes including the significance of chemotherapy on tumors.
Results: There was statistically significant overexpression of KIF14 and E2F3 mRNA in tumors compared with control
retinas (p<0.0001). Further, E2F3 also showed a significant overexpression compared to RB cell lines (p=0.01).
Immunohistochemistry confirmed KIF14 and E2F3 protein overexpression in tumor cells. KIF14 had significant mRNA
overexpression with older age (p=0.01) in presenting patients and in unilateral RB patients (p=0.04). Chemotherapytreated
tumors showed a significant decrease in KIF14 and E2F3 expression compared to untreated tumors (p<0.01 and
0.001, respectively).
Conclusions: This report confirms significant mRNA overexpression of KIF14 and E2F3 together in a large cohort of
RB tumors. The decreased expression in chemotherapy treated cases needs further validation in a large chemotherapytreated
cohort.
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