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The serine/threonine protein kinasePknI controls the growth of Mycobacterium tuberculosis upon infection

Gopalaswamy, Radha and Narayanan, Sujatha and Chen, B and Jacobs, W R and Av-Gay, Yossef (2009) The serine/threonine protein kinasePknI controls the growth of Mycobacterium tuberculosis upon infection. FEMS Microbiology Letters, 295 (1). pp. 23-29. ISSN 0378-1097; eISSN: 1574-6968

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Abstract

The protein kinase PknI is one of 11 functional serine/threonine protein kinases in Mycobacterium tuberculosis. Specialized transduction was performed to create a null mutant in the pknI gene. The resulting mutant was used to determine the role of PknI in M. tuberculosis growth and infectivity. The pknI mutant grows better under acidic pH and limited oxygen availability. We observed a modest increased growth of pknI mutant within macrophages during an in vitro infection and a hypervirulence phenotype in severe combined immunodeficiency mice. The internal signals used to activate PknI are most likely the host-associated signals such as low pH associated with limited oxygen availability. Thus, we have shown that PknI plays a role in sensing the host macrophage’s environment and translating it to slow the growth of M. tuberculosis within the infected host.

NIRT Creators:
NIRT CreatorsEmail
Item Type: Article
Additional Information: DOI:10.1111/j.1574-6968.2009.01570.x
Uncontrolled Keywords: tuberculosis; PknI; STPKs; macrophage; infection
Subjects: Tuberculosis > Laboratory Research > Immunological
Tuberculosis
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 14 Nov 2017 07:23
Last Modified: 14 Nov 2017 07:23
URI: http://eprints.nirt.res.in/id/eprint/954

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