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Development of DNA probes for M. tuberculosis

Narayanan, Sujatha and Sahadevan, R and Ramanujam, S and Prabhakar, R and Narayanan, P R (1992) Development of DNA probes for M. tuberculosis. Indian Journal of Tuberculosis, 40 (2). pp. 99-105. ISSN 0019-5705

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Abstract

Attempts were made to develop DNA probes for M. tuberculosis. Random library of M. tuberculosis was constructed in plasmid pGEM -4. Selection of recombinant clones was made by hybridisation with 32P labelled M. tuberculosis probe. Ten recombinant clones were selected on the basis of strong signals from the random library. These 10 clones named pTRC1-10 were subjected to tests for specificity and sensitivity. On this basis, pTRC4 was chosen and this is also, useful in restriction fragment length polymorphism (RFLP) studies.

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Item Type: Article
Subjects: Tuberculosis > Laboratory Research > Immunological
Divisions: Basic Science Research > Immunology
Depositing User: Dr. Rathinasabapati R
Date Deposited: 12 Sep 2013 09:29
Last Modified: 14 Mar 2016 07:24
URI: http://eprints.nirt.res.in/id/eprint/330

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