5′ Regulatory and 3′ Untranslated Region Polymorphisms of Vitamin D Receptor Gene in South Indian HIV and HIV–TB Patients

Alagarasu, K and Selvaraj, P and Swaminathan, S and Narendran, G and Narayanan, P R (2009) 5′ Regulatory and 3′ Untranslated Region Polymorphisms of Vitamin D Receptor Gene in South Indian HIV and HIV–TB Patients. Journal of Clinical Immunology, 29 (2). pp. 196-204. ISSN 0271-9142; eISSN: 1573-2592

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Abstract

Introduction VitaminD receptor (VDR) gene polymorphisms in the 5′ regulatory region (Cdx2 and A-1012G), coding region (FokI), and 3′ untranslated region (UTR; BsmI, ApaI, and TaqI) were studied to find out whether these polymorphisms are associated with susceptibility to or protection against HIV-1 and development of tuberculosis (TB) in human immunodeficiency virus (HIV)-1-infected patients. Study Subjects and Methods The study was carried out in 131 HIV patients without TB (HIV+ TB−) and 113 HIV patients with TB (HIV+ TB+; includes 82 patients with pulmonary TB (HIV+ PTB+) and 31 with extra pulmonary TB), 108 HIV-negative pulmonary TB patients (HIV− PTB+), and 146 healthy controls. Results Among the 5′ regulatory and coding region polymorphisms, significantly increased frequency of G/A genotype of Cdx-2 was observed in HIV+ TB− group compared to controls (p=0.012, odds ratio (OR) 1.89 95% confidence interval (CI) 1.14–3.15). In the 3′ UTR genotypes, a decreased frequency of b/b genotype of BsmI in total HIV patients (p=0.014, OR 0.54 95% CI 0.32– 0.89) and increased frequencies of A/A genotype of ApaI in HIV+ TB+ patients (p=0.041, OR 1.77 95% CI 1.02–3.06) and t/t genotype of TaqI in HIV+ PTB+ patients (p=0.05, OR 2.32 95% CI 0.99–5.46) were observed compared to controls. Haplotype analysis revealed significantly increased frequencies of 3′ UTR haplotype B-A-t in HIV+ TB+ and HIV+ PTB+ groups (Pc=0.030, OR 1.75 95% CI 1.14–2.66) and decreased frequencies of b-A-T haplotype in total HIV patients (Pc=0.012, OR 0.46 95% CI 0.27– 0.77), HIV+ TB− (p=0.031 OR 0.48 95% CI 0.25–0.89), and HIV+ PTB+ groups (Pc=0.04, OR 0.47 95% CI 0.23– 0.89) compared to controls. Conclusions The results suggest that VDR gene 3′ UTR haplotype b-A-T may be associated with protection against HIV infection while B-A-t haplotype might be associated with susceptibility to development of TB in HIV-1-infected patients.

Affiliation:	NIRT
Item Type:	Article
Additional Information:	DOI 10.1007/s10875-008-9234-z
Uncontrolled Keywords:	Vitamin D receptor, polymorphisms, HIV-1, tuberculosis, HIV–TB
Subjects:	Tuberculosis > Laboratory Research > Immunological HIV Tuberculosis
Divisions:	Basic Science Research > Immunology
Depositing User:	Dr. Rathinasabapati R
Date Deposited:	08 Aug 2017 11:36
Last Modified:	22 Apr 2022 06:17
URI:	http://eprints.nirt.res.in/id/eprint/940

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